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It is also important that you do not have "Only Web Colors" selected in the Color Picker window. NOTE: the background surrounding your specimen may not be exactly black, so it is important to use the eye dropper tool rather than simply creating a black background. Use the eye dropper tool to select the dark background surrounding your specimen and the background will change to duplicate that color.

Select Solid Color from the background list and a white background will appear, along with a color selection box and an eye dropper tool. Select the black background layer and click on the black and white circle icon to create a new background. Open the Layers window and you will see that you now have two layers. Right-click, create new layer using layer via cutīefore proceeding, make sure that your colors are set to white and black in your tool bar, with white on top. Try to avoid dust particles and other crud that you don’t want in your final image. Using the lasso tool (l) draw a line around the specimen. Now you image resolution has been set to work with the Draw Scale script that you will run later. I won’t look like anything happened, but the line you drew previously will disappear. Browse to the folder containing your scripts (you only have to do this once and Photoshop will remember for the next time) and double-click ScaleImage.jsx. Be sure to start on the left side of a line and end on the left side of a line for accuracy.Ĭlick Alt+F1 and browser window will open. Using the line tool (u), draw a 15mm line (hold the shift key as you draw in order to keep straight line).

Select Ok, and the image will align itself so that the scale bar is now straight. Click F1 and the Rotate Canvas window will open. With the ruler (i), draw a line along the edge of the scale bar. The ruler tool is often hidden under the eye dropper. Select the ruler tool to draw a line along edge of scale bar, then click f1 and straighten. Using the zoom (z) tool, zoom to the scale (hold alt key to zoom out hold space bar to move image ) Straighten Scale Bar (if scale bar is straight already, proceed to step 3.) Next shift the right arrow to the left in order to correct for white. Shift the left arrow to the right in order to make the background black (be careful not to obscure any dark details on your specimen – this may require zooming in on the specimen first, before setting levels). Z:\DocLib\code_and_scripts\PhotoshopScripting\NSF_Scripts\Production\Ĭlick Ctrl+L and the levels window will open. Open Specify and run a query to check to be sure that the number and the label information match up with the data in Specify.įYI – the Photoshop scripts that you will need for this process are kept in the DocLib on the network drive. Open an image in photoshop - make sure colors in the tool bar are set to white and black, with white on top. To check go to Edit -> Preferences -> Units & Rulers, and set units to mm. If you have not already done this, go to Liath's Protocol - Creating New HotkeysĪlso, you will need to have your units set to mm. Before using this protocol you will need to create a few hotkeys.